Coding

Part:BBa_K3385040:Design

Designed by: Lucas Levassor   Group: iGEM20_DTU-Denmark   (2020-10-12)


Gene_glaA


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal PstI site found at 879
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 1756
    Illegal PstI site found at 879
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 734
    Illegal BamHI site found at 1714
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal PstI site found at 879
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal PstI site found at 879
    Illegal NgoMIV site found at 1974
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 262
    Illegal BsaI site found at 400
    Illegal BsaI site found at 1089
    Illegal BsaI.rc site found at 1582


Design Notes

We chose glucoamylase for our project because it is industrially relevant compared to fluorescent proteins.

Source

Obtained through PCR amplification from Aspergillus niger.

References